| 摘要 |
PROBLEM TO BE SOLVED: To generate a stable complex and at the same time achieve a fluorescent labeling method by utilizing the complex having a long fluorescent life and intense fluorescence by formingβ-diketone structure shown by a specific formula for forming a rare earth ion and a complex in a molecule and a structure for containing a bonding group with a substance to be labeled and a hydrophilic group. SOLUTION: A reagent is composed by containingβ-diketone structure that is shown by formula (in which, R2 and R3 indicate respectivelyβ-diketone group that is bonded with the ortho or meta position of benzene ring) for forming at least a rare earth ion and a complex in a molecule, the bonding group with a substance to be labeled, and a hydrophilic group. At least two or fourβ- diketone structures are included and operate as a tetradentate or octadentate ligand for the rare earth ion. In the molecule, a hydrophilic part and a bonding group for labeling an organism substance are contained. The bonding group with the substance to be labeled reacts with a substituent to form a covalent bonding group. The hydrophilic group introduces hydrophilic property for easily adjusting, storing, or using a labeling reagent in a water solution.
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