SUBSTRATE AND APPARATUS FOR FLUOROMETRIC ANALYSIS

摘要

PROBLEM TO BE SOLVED: To provide a substrate and apparatus for fluorometric analysis in which fluorometric analysis can be conducted while prolonging the lifetime of fluorescence from a sample and enhancing S/N ration. SOLUTION: Since a substrate 1 for fluorometric analysis comprises a silicon substrate 2 and a silicon oxide film 3 of 10 nm-2.5μm thick formed on the side of the silicon substrate 2 to be bonded with a sample, quenching action of fluorescence due to the silicon substrate 2 is suppressed and the effect of noise light being emitted from the silicon oxide film 3 is reduced thus conducting fluorometric analysis efficiently. Furthermore, since the substrate for fluorometric analysis comprises a fluorescent region having the silicon oxide film 3 and a quenching region only of the silicon substrate 2, measurement efficiency can be enhanced significantly especially for a trace sample, e.g. a single molecule.