Production of microorganisms that overproduce pantothenic acid, useful as vitamin in e.g. foods or medicines, by overexpressing sequences that encode ketopantothenate reductase

摘要

Production, and improvement, of panthothenic acid (I)-producing microorganisms by amplifying (particularly overexpressing) sequences (I) that encode ketopanthoate reductase (KPR), specifically the panE gene, either individually or together. Optionally the ilvC gene is also amplified. Independent claims are also included for the following: (1) plasmid vectors pFE80, pFE65 and pFE32, deposited, in Escherichia coli K12 strain MG1655, as DSM 12414, 12382 and 12413, respectively; (2) the E. coli K12 strain FE6 which is resistant to valine; (3) the E. coli strain FE7 in which the avtA gene is exchanged for a avtA::aadB fragment (deposited as DSM 12380); (4) a host microorganism (E. coli or Corynebacterium) that contains one of the specified plasmids and is optionally resistant to one or more metabolites and/or antimetabolites; C. glutamicum ATCC13032/pFE91, where pFE91 includes the C. glutamicum ilvC gene; and (5) production of (I) by culturing any of the specified microorganisms and isolation of (I) from medium or cells.