| 摘要 |
The invention concerns fluorescent probes used in multicolour in situ fluorescent hybridisation methods, and principally chromosomal painting. The probes designed for marking a chromosome, are such that they consist of a set of DNA segments more represented in certain chromosomal bands and are obtained by IRS-PCR amplification from said chromosomes using PCR primers specific of the repeated and dispersed DNA sequences Alu and LINE. The invention further concerns methods for producing said probes, multicolour FISH methods capable of using said probes and diagnosis kits comprising them. The invention also concerns combinations of fluorophores and optical filters.
|
