| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new gene which is composed of the gene coding for endoglucanase containing a specific amino acid sequence and is useful in the production of the above enzyme contributing to the effective utilization or the like of cellulose-based biomass or the improvement due to the elucidation of the specific characteristic of the enzyme. SOLUTION: This gene is a new DNA comprising the base sequence, which codes for the amino acid sequence of formula I (wherein, X is not contained or is the leader sequence containing formula II) showing endoglucanase activity or the amino acid sequence that is obtained by adding, deleting or substituting one or plural amino acids to this amino acid sequence and shows endoglucanse activity, and is useful in the biochemical analysis and high efficient production of this enzyme contributing to the effective utilization of cellulose-based biomass and in the improvement of the specific characteristic due to the elucidation of the specific characteristic of the enzyme or the gene modification. This DNA is obtained by incubating Corticium rolfsii to recover the microbial cell body, extracting the RNA, preparing a cDNA library by using this RNA and then screening out it with an antiendoglucanase serum. |
