Method of detecting p53-specific antibodies

摘要

PCT No. PCT/EP93/02666 Sec. 371 Date Sep. 15, 1994 Sec. 102(e) Date Sep. 15, 1994 PCT Filed Sep. 30, 1993 PCT Pub. No. WO94/08241 PCT Pub. Date Apr. 14, 1994The invention relates to a method of detecting p53-specific antibodies in body fluids wherein support-bound p53 and/or support-bound fragments thereof comprising binding regions for p53-specific antibodies are incubated with body fluids and the specific antibodies (a) bound to p53 and/or said fragments are allowed to react with labelled antibodies (b) directed against antibodies (a), or with unlabelled antibodies (b) while the latter are allowed to react with labelled antibodies (c) directed against antibodies (b), with the labelling being non-radioactive in either case. The invention further relates to a kit useful therefor. Also, the invention relates to p53 fragments and DNA sequences coding for them, said fragments comprising binding regions for p53-specific antibodies, as well as to methods of preparing them.