发明名称 |
Methods for screening for inhibitors of the transcription-enhancing activity of the X protein of hepatitis B virus |
摘要 |
Disclosed is a method for screening for inhibitors of Hepatitis B Virus pX activity. The method involves contacting a test compound with (I) the pX protein of HBV, (ii) a transcription factor comprising the bZIP domain, or fragments that comprise a minimal a bZIP domain, and (iii) an oligoduplex comprising a target DNA sequence of the transcription factor to form a test mixture. After incubating the test mixture under appropriate conditions and for a sufficient time to allow pX-mediated dimerization and DNA binding of the transcription factor to occur, the level of DNA binding of the transcription factor in each test mixture is determined. A test compound is considered to be any compound that causes a decrease in the level of DNA binding in the test mixture relative to the level of DNA binding in control mixtures.
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申请公布号 |
US6051373(A) |
申请公布日期 |
2000.04.18 |
申请号 |
US19940351659 |
申请日期 |
1994.12.07 |
申请人 |
SCRIPTGEN PHARMACEUTICALS, INC.;UNIVERSITY OF MASSACHUSETTS MEDICAL CENTER |
发明人 |
GREEN, MICHAEL R.;PERINI, GIOVANNI;LILLIE, JAMES |
分类号 |
G01N33/576;C07K14/02;C12N15/00;C12N15/09;C12N15/67;C12Q1/18;C12Q1/68;C12R1/92;G01N33/15;(IPC1-7):C12Q1/70;G01N33/53;G01N33/566 |
主分类号 |
G01N33/576 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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