发明名称 |
Oligonukleotid-screeningsassay |
摘要 |
The invention features a method for assaying the ability of an oligonucleotide to form a hybrid with a target nucleic acid sequence. The featured assay comprises the following steps: (a) contacting a test sample containing a target nucleic acid sequence with an oligonucleotide; (b) treating the test sample with a duplex-altering agent; (c) contacting the treated test sample with a labeled probe under stringent hybridization conditions, wherein the probe can hybridize to non-altered target nucleic acid sequence such that the probe label is protected from subsequent chemical inactivation but the probe cannot hybridize to altered target nucleic acid sequence in a manner which would protect the probe label from subsequent chemical inactivation; and (d) measuring the hybridization of the probe to non-altered target nucleic acid sequence by measuring the amount of protected label. Also described is an assay to measure the ability of a ribozyme to cleave a target nucleic acid sequence, and an assay to measure the ability of an agent to alter nucleic acid. |
申请公布号 |
DK0639648(T3) |
申请公布日期 |
2000.04.17 |
申请号 |
DK19940305271T |
申请日期 |
1994.07.19 |
申请人 |
GEN-PROBE INCORPORATED |
发明人 |
KACIAN, DANIEL L.;VELARDE, JORGE JR;NELSON, NORMAN C. |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|