发明名称 |
Separation and concentration systems based on soluble oligosaccharide binding domains |
摘要 |
A compound having a polysaccharide binding domain such as contained by a cellulose and essentially lacking in polysaccharidase activity is purified from other ingredients in a mixture using an affinity partition system. A mixture containing the compound is contacted with a system containing as a first phase an aqueous solution of oligosaccharide polymer such as cellulose and as a second phase a solution of a polymer such as a poly(ethylene glycol)-poly(propylene glycol) copolymer. The compound petitions into the first phase and binds to the oligosaccharide polymer, preferably with a Ka of 103 to 107, to form a complex. The complex is collected, and the compound is dissociated from the oligosaccharide polymer. The compound may be formed of a non-peptide chemical moiety or a peptide moiety linked to a polypeptide having the polysaccharide binding domain. The compound may also be a fusion polypeptide containing the polysaccharide binding domain linked through a protease recognition sequence to a macromolecule such as an enzyme, a hormone or an antibody. The macromolecule can be removed by using a protease to cleave the recognition sequence. Another partition system contains the oligosaccharide polymer and a phase separation inducing agent such as a sulfate or citrate salt that induces separation to produce different phases. |
申请公布号 |
AU718247(B2) |
申请公布日期 |
2000.04.13 |
申请号 |
AU19960066805 |
申请日期 |
1996.07.24 |
申请人 |
UNIVERSITY OF BRITISH COLUMBIA |
发明人 |
CHARLES A. HAYNES;PETER TOMME;DOUGLAS G KILBURN |
分类号 |
C12N15/09;A61K39/39;C07K1/14;C07K14/55;C08B15/00;C08B37/08;C08H8/00;C12N9/16;C12N9/38;C12N9/42;C12N11/12;C12P19/02;C12Q1/56;D21H17/00;G01N33/531 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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