INTEGRAL MULTILAYER ANALYTICAL ELEMENT FOR ASSAYING SULFATE-CONJUGATED BILE ACID

摘要

An integral multi-layer analytical element for analyzing bile acid sulfate comprising a support member, a reagent layer on said support member and a porous developing layer on said reagent layer, at least one of the reagent layer and developing layer containing bile acid sulfate sulfatase, 3 beta -hydroxysteroid dehydrogenase and a combination of thio-NAD<+> and reduced NAD or reduced NADP, in which the reagent layer contains a water-soluble polymer, a buffer and at least one component selected from the group consisting of sugar alcohols and Mn<2+>, which element suppresses the increase of blank values and prevents agglomeration of the enzyme during the preparation of a solution for forming the reagent layer.