| 摘要 |
The molecular cloning and characterization of a novel human immunodeficiency virus type 2 (HIV-2), designated HIV-2ROD, is described. A recombinant lambda phage genomic library was screened with an HIV-2-specific probe to identify overlapping subgenomic clones. Fragments of these lambda phage clones were subcloned into a suitable vector to reconstitute the complete HIV-2ROD genome. The complete nucleotide sequence of this proviral clone was ascertained and the following genes and gene products identified: gag (including p16, p26, and p12), pol, vif, vpr, vpx, env, tat, rev, and nef. These gene products will be useful, inter alia, in in vitro diagnostic methods and kits for the detection of HIV-2-specific antisera.
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