发明名称 |
Articular cartilage xenografts |
摘要 |
The invention provides an article of manufacture comprising a substantially non-immunogenic articular cartilage xenograft for implantation into humans. The invention further provides a method for preparing an articular cartilage xenograft by removing at least a portion of an articular cartilage from a non-human animal to provide a xenograft; washing the xenograft in saline and alcohol; subjecting the xenograft to at least one treatment selected from the group consisting of exposure to ultraviolet radiation, immersion in alcohol, ozonation, freeze/thaw cycling, and optionally to chemical crosslinking. In addition to or in lieu of the above treatments, the methods include a cellular disruption treatment and either digestion of the carbohydrate moieties of the xenograft with a glycosidase in a range of about 1 mU/ml to about 1000 U/ml or glycosidase digestion followed by treatment for sialylation. The invention also provides articles of manufacture produced by one or more of the above-identified methods of the invention. The invention further provides an articular cartilage xenograft for implantation into a human including a portion of an articular cartilage from a non-human animal, wherein the portion includes extracellular matrix and substantially only dead cells. The matrix and dead cells have substantially no surface alpha -galactosyl moieties and have sialic acid linked to at least a portion of surface carbohydrate moieties. Each of the xenografts of the invention is substantially non-immunogenic and has substantially the same mechanical properties as the respective native articular cartilage.
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申请公布号 |
US6049025(A) |
申请公布日期 |
2000.04.11 |
申请号 |
US19980036098 |
申请日期 |
1998.03.06 |
申请人 |
STONE, KEVIN R.;GALILI, URI |
发明人 |
STONE, KEVIN R.;GALILI, URI |
分类号 |
A61F2/30;A61F2/38;A61L27/36;(IPC1-7):A61F2/28 |
主分类号 |
A61F2/30 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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