HIGH THROUGHPUT ASSAY METHOD FOR ENZYMES WHICH METABOLICALLY HYDROLYZE NUCLEOSIDE TRIPHOSPHATES AND AN ASSAY SYSTEM THEREFOR

摘要

The present invention is drawn to a high throughput assay method of monitoring the activity of enzymes which hydrolyze NTPs as cosubstrates by forcing the reaction to run in the metabolically reverse direction and quantitating the NTP synthesized by the target enzyme. The present invention is further drawn to assay systems which use the assay method.