TREX, A NOVEL GENE OF TRAF-INTERACTING EXT GENE FAMILY AND DIAGNOSTIC AND THERAPEUTIC USES THEREOF

摘要

This invention provides an isolated nucleic acid molecule encoding a Tumor necrosis factor Receptor-Associated Factor (TRAF) protein-interacting hereditary multiple extoses (TREX) protein. This invention also provides vectors comprising the isolated nucleic acid molecule encoding a TREX protei n. This invention further provides a purified TREX protein and antibodies thereto. This invention provides oligonucleotides comprising a nucleic acid molecule of at least 15 nucleotides capable of specifically hybridizing with a unique sequence included within the sequence of an isolated nucleic acid molecule encoding TREX protein. This invention provides an antisense oligonucleotide comprising a sequence capable of specifically hybridizing wi th a unique sequence included within a genomic DNA molecule encoding a Tumor necrosis factor Receptor-Associated Factor (TRAF) protein-interacting hereditary multiple extoses (TREX) protein. This invention provides a monoclonal antibody directed to an epitope of a TREX protein. This invention provides methods of inhibiting TREX protein interaction with a TRAF protein; of inhibiting overexpression of TREX protein; of inhibiting growth of a tumo r; of treating abnormalities in a subject associated with overexpression of TRE X. This invention provides pharmaceutical compositions comprising oligonucleotides effective to prevent overexpression of a TREX protein or antibodies effective to block binding of a TREX protein to a TRAF protein; screening for compounds which inhibit TREX protein and TRAF protein binding; of detecting predispositions to cancers comprising TREX mutations; and of diagnosing cancer comprising TREX mutations.