发明名称 |
Modulating Robo: ligand interactions |
摘要 |
Disclosed are methods and compositions for identifing agents which modulate the interaction of Robo and a Robo ligand and for modulating the interaction of Robo and a Robo ligand. The methods for identifying Robo:ligand modulators find particular application in commercial drug screens. These methods generally comprise (1) combining a Robo polypeptide, a Slit polypeptide and a candidate agent under conditions whereby, but for the presence of the agent, the Robo and Slit polypeptides engage in a first interaction, and (2) determining a second interaction of the Robo and Slit polypeptides in the presence of the agent, wherein a difference between the first and second interactions indicates that the agent modulates the interaction of the Robo and Slit polypeptides. The subject methods of modulating the interaction of Robo and a Robo ligand involve combining a Robo polypeptide, a Slit polypeptide and a modulator under conditions whereby, but for the presence of the modulator, the Robo and Slit polypeptides engage in a first interaction, whereby the Robo and Slit polypeptides engage in a second interaction different from the first interaction. In a particular embodiment, the modulator is dominant negative form of the Robo or Slit polypeptide.
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申请公布号 |
US6046015(A) |
申请公布日期 |
2000.04.04 |
申请号 |
US19980191647 |
申请日期 |
1998.11.13 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
GOODMAN, COREY S.;KIDD, THOMAS;BROSE, KATJA;TESSIER-LAVIGNE, MARC |
分类号 |
C12N15/09;A61K38/00;A61K38/17;A61P43/00;C07K14/47;C07K14/705;C07K16/18;C12N15/12;G01N33/15;G01N33/50;G01N33/566;G01N33/68;(IPC1-7):G01N33/53;C07K7/08;C12N5/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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