发明名称 |
SYNTHESIS OF VANILLIN FROM A CARBON SOURCE |
摘要 |
A bioengineered synthesis scheme for the production of vanillin from a carbon source is provided. The bioconversion methods of the present invention comprise the steps of microbe-catalyzed conversion of a carbon source to vanillic acid followed by enzyme-catalyzed reduction of the vanillic acid to produce vanillin. As shown in the synthesis scheme of Figure 2, the microbe-catalyzed conversion step of the present invention requires five enzymes which are provided by a recombinant microbe. In a preferred embodiment, the recombinant microbe is Escherichia coli designed to cause dehydration of 3-dehydroshikimic acid and regioselective methylation of the resulting protocatechuic acid. The enzyme-catalyzed reduction step of the present invention comprises the reduction of vanillic acid to vanillin by aryl-aldehyde dehydrogenase. |
申请公布号 |
WO0017319(A2) |
申请公布日期 |
2000.03.30 |
申请号 |
WO1999US21323 |
申请日期 |
1999.09.16 |
申请人 |
BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIVERSITY;FROST, JOHN, W. |
发明人 |
FROST, JOHN, W. |
分类号 |
C12N9/02;C12N15/52;C12P7/24 |
主分类号 |
C12N9/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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