TRANSFECTED CELLS AND METHODS FOR TREATING DIABETES
摘要
The present invention provides an isolated population of cells containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsuling cleavage site to produce insulin. The invention also provides an isolated population of cells which further express a hexosamine synthetic pathway enzyme. The invention additionally provides vectors containing an expressible nucleic acid encoding proinsulin containing a proinsulin cleavage site and a glucose-regulated expressible nucleic acid encoding a protease capable of cleaving the proinsulin cleavage site to produce insulin. The invention further provides a method of treating or preventing diabetes by implanting into an individual cells coexpressing proinsulin containing a proinsulin cleavage site and a glucose-regulated protease capable of cleaving the proinsulin cleavage site to produce insulin.
申请公布号
WO9963101(A9)
申请公布日期
2000.03.30
申请号
WO1999US11970
申请日期
1999.06.01
申请人
UNIVERSITY OF WASHINGTON;OSBORNE, WILLIAM, R., A.;RAMESH, NAGARAJAN