| 摘要 |
<p>A method is disclosed for immobilizing enzymes for use in catalyzing organic reactions, in which the enzyme is imbibed into a dehydrated hydrocolloid polymer gel bead which has a network structure capable of swelling in aqueous media. The resulting enzyme-laden bead may then itself be dehydrated for eventual use in such organic reactions. The use of the enzyme laden beads as a catalyst for organic reactions is demonstrated in the transesterification reaction of N-acetyl-L-phenylalanine ethyl ester with propanol to N-acetyl-L-phenylalanine propyl ester in an octane solvent using subtilisin Carlsberg enzyme.</p> |
