| 摘要 |
The invention relates to a method for detecting nucleotide sequences or nucleotides-binding proteins in a sample, comprising of bringing the sample into contact with a standard solution which contains as components one or more known probes in an incubation mixture, in order to enable complexing between the probe and the nucleotide sequence and/or the nucleotide-binding protein to be detected in the sample, and determining whether complexing has taken place by comparing the molecular weight of the probe(s) from the incubation mixture with the molecular weight of the probe(s) in the standard solution, wherein determining whether complexing has taken place is brought about by column-chromatographic separation of the components of the incubation mixture, separation in the same manner of the components of the standard solution and comparing the elution patterns of both separations, wherein a change in the elution speed of one or more probe(s) in the incubation mixture relative to the elution speed of one or more probe(s) in the standard solution indicates the presence of complexing. |
