发明名称 |
Amplification of dna with a control sequence differing in gc content |
摘要 |
A method for amplifying a target DNA sequence, said method comprising amplifying said sequence in the presence of: a) a nucleic acid polymerase; b) at least one primer capable of hybridising to said target polynucleotide; c) an internal control sequence to which said primer is capable of hybridising and which is of similar length to the target DNA sequence but with a different percentage GC content; and d) label means for detecting the hybridisation of nucleic acids in the reaction; and detecting the hybridisation of the target and control sequences at different temperatures. |
申请公布号 |
AU5641099(A) |
申请公布日期 |
2000.03.27 |
申请号 |
AU19990056410 |
申请日期 |
1999.09.03 |
申请人 |
THE SECRETARY OF STATE FOR DEFENCE |
发明人 |
MARTIN ALAN LEE;GALE BRIGHTWELL |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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