| 摘要 |
Disclosed are compositions and methods of use of the promoter for prostate specific transglutaminase (SEQ ID NO:15). The invention relates particularly to isolated nucleic acids and vectors comprising the sequence of this promoter. The invention also relates to methods of therapeutic treatment for prostate cancer or benign prostatic hyperplasia (BPH) utilizing this promoter. Described are means for the isolation and identification of transcriptional factors and other DNA-binding proteins that regulate promoter transcriptional activity, identification of regulatory elements within the promoter and construction of deletion mutants containing specific subsets of these regulatory elements, identification of small molecule ligands that bind to and inhibit or activate the identified transcriptional factors and other DNA-binding proteins, construction of vectors containing the prostate specific transglutaminase promoter operatively linked to genes of use in the treatment of prostate cancer or BPH, and methods for treatment of prostate cancer or BPH by administration of such vectors to patients with prostate cancer or BPH. Further described are methods for treatment of prostate cancer or BPH by administration of small molecule ligands that bind to and inhibit or activate transcriptional factors or other DNA-binding proteins that regulate the activity of this promoter. |
