发明名称 |
Stimulation of angiogenesis via enhanced endothelial expression of syndecan-4 core proteins |
摘要 |
The present invention provides tangible means and methods for stimulation of angiogenesis via enhanced endothelial expression of core proteins having a syndecan-4 cytoplasmic region intracellularly. The tangible means include a prepared DNA sequence fragment having separate and individual DNA sequenced portions coding for an heparan sulfate binding extracellular domain, a central transmembrane domain, and a cytoplasmic domain coding for the syndecan-4 polypeptide. The prepared DNA sequence unitary fragment may be delivered to endothelial cells in-situ, both under in-vivo and/or in-vitro conditions, using suitable expression vectors including plasmids and viruses. The resulting transfected endothelial cells overexpress heparan sulfate binding, core proteins; and the resulting overexpression of these proteoglycan entities causes stimulation of angiogenesis in-situ. |
申请公布号 |
AU5679499(A) |
申请公布日期 |
2000.03.27 |
申请号 |
AU19990056794 |
申请日期 |
1999.08.18 |
申请人 |
BETH ISRAEL DEACONESS MEDICAL CENTER |
发明人 |
MICHAEL SIMONS;RUDIGER VOLK;ARIE HOROWITZ |
分类号 |
A61K48/00;C07K14/47;C07K14/705;C12N15/08 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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