Method of inducing transduction with retroviral assistance

摘要

he disclosure provides a retroviral packaging system, in which retroviral packaging constructs, and packaging vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retroviruses are produced in infected cells. The methods include the use of the retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. Said method is useful for the rapid production of high titer viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.