发明名称 |
Method for monitoring the temperature of a biochemical reaction |
摘要 |
A method of monitoring the temperature of a biochemical reaction such as an amplification reaction is described. The method comprises effecting the reaction in the presence of a fluorescently labeled temperature probe DNA sequence which comprises a double stranded region which denatures at a predetermined temperature, the fluorescent label of said temperature probe sequence being arranged so that a detectable signal occurs at the point at which denaturation of said region takes places; and monitoring fluorescence from said reaction mixture so as to determine when said predetermined temperature has been reached. |
申请公布号 |
AU5640999(A) |
申请公布日期 |
2000.03.27 |
申请号 |
AU19990056409 |
申请日期 |
1999.09.03 |
申请人 |
THE SECRETARY OF STATE FOR DEFENCE |
发明人 |
MARTIN ALAN LEE;GALE BRIGHTWELL |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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