PROCESS FOR MAKING POLYCARBOXYLIC ACIDS

摘要

Aliphatic polycarboxylic acids are made by a process comprising the steps of : (1) fermenting a beta-oxidation blocked C. tropicalis cell wherein bo th copies of the chromosomal POX5 gene and the chromosomal POX4A and POX4B gene s are disrupted in a culture medium comprised of a nitrogen source, an organic substrate and a cosubstrate wherein the substrate is an unsaturated aliphati c compound having at least one internal carbon-carbon double bond and at least one terminal methyl group, a terminal carboxyl group and/or a terminal functional group which is oxidizable to a carboxyl group by biooxidation; (2 ) reacting the product of step (1) with an oxidizing agent to produce one or more polycarboxylic acids.