| 摘要 |
The present invention provides methods which generate a polypeptide that is secreted into the urine by using the uromodulin gene promoter, or other promoters from genes whose products are specifically expressed in the kidney, to direct the expression of the secreted polypeptide. Also disclosed is a transgenic animal secreting a recombinant polypeptide into its urine, as well as a method for detecting such an animal while the animal is in utero. Also disclosed are methods for generating a polypeptide that is secreted into the conditioned media of cultured kidney cells transformed with a polypeptide-encoding nucleic acid sequence that is operably linked to a uromodulin gene promoter, or other kidney specific gene promoter.
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