发明名称 |
Method for chemical synthesis of oligonucleotides |
摘要 |
The present invention provides a practical method capable of chemically synthesizing a 100-mer or more long-chain oligonucleotide easily and reliably and a novel compound used in said method. The present invention relates to a method for chemical synthesis of an oligonucleotide by the phosphoroamidite method, which comprises preparing a base moiety-unprotected nucleoside phosphoroamidite from a base moiety-unprotected nucleoside by use of an imidazole trifluoromethanesulfonate represented by the following chemical formula, and coupling said base moiety-unprotected nucleotide phosphoroamidite in a predetermined order to chemically synthesize an oligonucleotide consisting of a specific nucleotide sequence, as well as to an imidazole trifluoromethanesulfonate represented by the chemical formula.
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申请公布号 |
US6040439(A) |
申请公布日期 |
2000.03.21 |
申请号 |
US19980145973 |
申请日期 |
1998.09.03 |
申请人 |
JAPAN SCIENCE AND TECHNOLOGY CORPORATION |
发明人 |
HAYAKAWA, YOSHIHIRO;KATAOKA, MASANORI |
分类号 |
C07D233/58;C07D235/08;C07H1/00;C07H19/04;C07H21/00;(IPC1-7):C07H21/00 |
主分类号 |
C07D233/58 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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