发明名称 |
Process for the preparation of active somatotropin from inclusion bodies |
摘要 |
A process for the preparation of biologically active somatotropin from inclusion bodies of a recombinant host cell containing an inactive form of said somatotropin protein comprises the steps of: (a) contacting the inclusion bodies with an aqueous alcohol solution at an alkaline pH to solubilize said protein; and (b) bringing the solubilized protein into contact with a mild oxidizing agent to refold and form intramolecular disulfide bonds between cysteine residues of said protein. |
申请公布号 |
AU5530399(A) |
申请公布日期 |
2000.03.21 |
申请号 |
AU19990055303 |
申请日期 |
1999.08.26 |
申请人 |
LG CHEMICAL LIMITED |
发明人 |
YONG JUN LEE;HONG KYUN LEE;KYUBOEM HAN |
分类号 |
C07K1/113;C07K14/61;C12N1/21 |
主分类号 |
C07K1/113 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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