| 摘要 |
A method for identifying 5-methylcytosine positions in the genomic DNA of a cell, cell line, tissue or individual is new and comprises: (1) chemically modifying the DNA by a treatment in which cytosine and 5-methylcytosine react differently, so that the cytosine and 5-methylcytosine positions will have different base-pairing properties; (2) amplifying a segment of the DNA by means of a polymerase reaction; (3) treating the same segment from at least one other cell, cell line, tissue or individual or from reference DNA as in (a) and (b); (4) forming heteroduplexes from the amplification products; and (5) detectably marking the heteroduplexes by a reaction specific for noncomplementary base pairs. Independent claims are also included for the following: (1) a kit for carrying out the method, comprising: (a) DNA from at least two maximally different individuals, tissues, cell lines or cells; and (b) reagents for detecting the variable methylation positions; (2) a kit for carrying out the method, comprising: (a) completely methylated and/or demethylated DNA; and (b) reagents for detecting methylated cytosines in any DNA sample.
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