POLYPEPTIDES CONTAINING MODIFIED, NON-NATURAL AMINO ACIDS AND METHODS OF PRODUCTION

摘要

A method for modifying a polypeptide includes providing a polypeptide containing at least one 3,4-dehydroproline and reacting the 3,4-dehydroproline with an epoxidation agent to form a polypeptide containing 3,4-epoxyproline. The method optionally further includes reacting the polypeptide containing 3,4-epoxyproline with a reducing agent to form a polypeptide containing a hydroxyproline. Polypeptides are provided which include an amino acid having an oxirane ring.