| 摘要 |
This invention provides a simple, inexpensive and high throughput assay method for the widely used herbicide, glyphosate, as an alternative to the currently used complex analytical methods. The invention provides a number of immunological reagents suitable for use in an immunoassay for glyphosate. The reagents include: (i) immunogens prepared by covalently linking glyphosate or glyphosine to a suitable carrier protein such as porcine thyroglobulin (TG) via the carbodiimide reaction, (ii) a phase coating conjugate prepared by covalently linking glyphosate to a suitable carrier protein such as BSA and (iii) antibodies to the above-mentioned immunogens. The invention also provides an immunoassay method with enhanced sensitivity (ppb level) for the analysis of glyphosate in environmental or biological samples. Methods for protein-hapten conjugate preparation, anti-glyphosate antibody production, and pre-assay derivatization of samples applicable to a variety of immunoassay formats are also provided.
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