METHOD FOR LEADING ADVENTITIOUS SHOOT FROM HYPOCOTYL SECTION OF SEED PLANT SEEDLING WITH HIGH FREQUENCY AND MANUFACTURING METHOD FOR REDIFFERENTIATED PLANT AND TRANSFORMED PLANT THEREOF

摘要

PURPOSE: A method for culturing a tissue is provided to be applied for multiplying a redifferentiated plant and transformed plant conveniently and certainly and to be used efficiently for mass-reproducing a plant and for developing a useful transformed plant. CONSTITUTION: A method is formed by:cutting a seed plant seedling except a certain part of the upper hypocotyl; and contacting the cut surface to a culturing bed for culturing under a light condition for inducing an adventitious shoot with high frequency. Herein, a seedling after 3 - 5 days from sprouting and containing one seed leaf or two leaves being cut to be halves is used for culturing. And the seed plant includes cucumber, Chinese matrimony vine, red pepper, melon, kidney bean, and stone-leek.