| 摘要 |
<p>Identification and speciation of bacteria of the Burkholderia cepacia complex in a sample can be accomplished by: a) obtaining nucleotide sequence information for the recA gene in bacteria of the Burkholderia cepacia complex found in the sample; and b) comparing the nucleotide sequence information obtained for the recA gene in bacteria of the Burkholderia cepacia complex found in the sample with a standard library of nucleotide sequence information comprising standard nucleotide sequence information for at least three species of bacteria of the Burkholderia cepacia complex. Preferably, the nucleotide sequence information is obtained by evaluation of restriction fragment length polymorphism (RFLP). Other techniques for obtaining sequence information can also be used, including base-by-base determination of the sequence of the region of interest, sequence-specific oligonucleotide hybridization probes, and ligation techniques. Universal primers which can be used for amplification of all known members of the Burkholderia cepacia complex, and genomovar-specific primers which can be used for selective amplification of the recA gene from bacteria of one genomovar provide alternative analytical modalities. Speciation of bacteria of the Burkholderia cepacia complex can be used as a basis for administration of a vaccine specific to the flagellin of the bacteria, since it is shown that this flagellin is conserved across members of genomovar III, subgroup RG-B.</p> |
