| 摘要 |
<p>1,135,987. Rubella vaccine; attenuating viruses. RECHERCHE ET INDUSTRIE THERAPEUTIQUES. 21 Oct., 1966, No. 47399/66. Heading A5B. Virulent Rubella virus is attenuated for use in a vaccine against Rubella, by subjecting it to at least fifteen serial passages on primary rabbit kidney tissue cultures. Preferably between 20 and 60 such passages are employed at a temperature of up to 36‹C, each passage being of from 3 to 6 days' duration. The virus may be obtained from throat swabs, urine or gargle samples from a clinical case of Rubella, and may be stored frozen, or inoculated into a suitable tissue culture, e.g. primary African green monkey kidney monolayers for isolation prior to attenuation. The rabbit kidney cell cultures are preferably from rabbits not more than 3 weeks old. After the final passage, the cells are separated from the supernatant fluid by filtration or centrifugation, leaving the virus in the medium. After titration of the virus strength and safety testing, the virus preparation is either freeze dried or stored frozen at -60‹C, and may be used as a vaccine by subcutaneous or intramuscular administration.</p> |
