发明名称 |
Method of expressing and secreting soluble extracellular domains of human gonadotropin hormone receptors |
摘要 |
The extracellular domain, or fragment thereof, of a gonadotropin glycoprotein hormone receptor is expressed and secreted in a soluble and functionally hormone-binding form. A recombinant baculovirus transfer vector is constructed to include a gene segment encoding the extracellular domain, or fragment thereof, of the glycoprotein hormone receptor joined in frame with a gene segment encoding a baculovirus signal peptide and operably linked to a baculovirus promoter. Recombinant baculovirus generated by transfection or co-transfection of insect cells are then used to infect insect host cells for the expression and secretion of soluble receptor.
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申请公布号 |
US6033903(A) |
申请公布日期 |
2000.03.07 |
申请号 |
US19950460576 |
申请日期 |
1995.06.02 |
申请人 |
APPLIED RESEARCH SYSTEMS, ARS HOLDING N.V. |
发明人 |
SISK, WILLIAM P.;CHENG, SHIRLEY VUI YEN;BUCKLER, DAVID ROGERS;PRENTICE, HOLLY LYNN |
分类号 |
C12N15/09;C07K1/22;C07K14/72;C12N5/10;C12N15/00;C12N15/12;C12N15/866;C12P21/02;C12R1/91;(IPC1-7):C12N15/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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