DIRECT OVIDUCT TRANSGENESIS

摘要

Methods for preparing transgenic avians which express exogenous protein substantially only in their oviducts are disclosed. Each of the methods comprises delivering nucleic acid expression cassettes directly to the oviducts of the avians. The exogenous protein expressed by the expression cassette is secreted into the lumen of the avian oviduct and secreted into the eggs of the transgenic avians. Methods for preparing eggs which contain exogenous protein, such as human interferon, and methods for the production of proteins are also disclosed. The methods for direct oviduct transgenesis may also be used to assess the suitability of expression cassettes or exogenous proteins for expression in the avian oviduct.