发明名称 |
NON-HUMAN ANIMAL HAVING A FUNCTIONALLY DISRUPTED SLP-76 GENE |
摘要 |
A non-human animal having somatic and germ cells in which at least one allele of an endogenous SLP-76 gene is functionally disrupted is provided. The animal may be heterozygous or, more preferably, homozygous for the SLP-76 gene disruption and is preferably a mouse. In homozygous animals, the percentage of peripheral T cells is substantially decreased compared to wildtype animals, whereas the percentage of B cells and macrophages in the periphery is substantially normal, indicating that SLP-76 disruption causes a profound block in T cell development. The animals of the invention can be used, for example, as controls to evaluate the efficacy of SLP-76 inhibitors and to identify disease conditions that can be treated with SLP-76 inhibitors. A transgenic non-human animal having a functionally disrupted endogenous SLP-76 gene but which has been reconstituted with an exogenous SLP-76 transgene (e.g., a human SLP-76 gene or a SLP-76 gene whose expression in targeted to a particular cell population) is also provided. An animal that has been reconstituted with a human SLP-76 gene can be used to identify agents that modulate human SLP-76 in vivo. Nucleic acid constructs for functionally disrupting an endogenous SLP-76 gene in a host cell, recombinant vectors including the nucleic acid construct, and host cells into which the nucleic acid construct has been introduced are also encompassed by the invention.
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申请公布号 |
WO9937756(A8) |
申请公布日期 |
2000.03.02 |
申请号 |
WO1999US01244 |
申请日期 |
1999.01.22 |
申请人 |
UNIVERSITY OF IOWA RESEARCH FOUNDATION |
发明人 |
KORETZKY, GARY, A.;CLEMENTS, JAMES, L.;WILLIAMSON, ROGER |
分类号 |
C07K14/47;C12N15/85;(IPC1-7):C12N15/00;C12N15/11;C12N15/63;C12N15/86;A01K67/00;G01N33/00 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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