| 摘要 |
<p>A method for inducing and enhancing mammalian mucosal immunity includes the steps of first administering to a mammal via a non-mucosal route a suitable amount of a priming vaccine composition which comprises a DNA sequence encoding an antigen of a pathogen under the control of regulatory sequences directing expression thereof in a mammalian cell, and subsequently, administering intranasally a boosting vaccine composition which comprises the same antigen in protein form or a DNA sequence encoding the same antigen. A method to reduce the anti-viral immune response to a recombinant viral vaccine includes the steps of administering a similar priming DNA vaccine composition that lacks any viral protein and subsequently administering as a boosting vaccine, a recombinant virus containing a DNA sequence encoding the same antigen as encoded by the DNA vaccine, wherein upon said recombinant virus vaccine administration, the immune response to the antigen is enhanced and the immune response to the recombinant virus is reduced.</p> |
