ALDEHYDE DEHYDROGENASE, ITS GENE, RECOMBINANT DNA AND PRODUCTION OF ALDEHYDE DEHYDROGENASE

摘要

PROBLEM TO BE SOLVED: To obtain an aldehyde dehydrogenase useful for enzymatically producing serine and cysteine. SOLUTION: This aldehyde dehydrogenase has the following physicochemical properties: (1) action: hydroxypyruvaldehyde + NAD+ → hydroxypyruvic acid + NADH + H; (2) substrate specificity: specifically acting on a 2-oxoaldehyde such as hydroxypyruvaldehyde, and slightly acting on a linear aldehyde with n=3-5; (3) optimum pH: about 10; (4) range of stable pH: 5-9; and (5) range of suitable temperature for action: 10-80 deg.C.