发明名称 |
Genetically engineered microorganisms and method for producing 4-hydroxybenzoic acid |
摘要 |
The present invention pertains to a method for economical biofermentative production of 4-hydroxybenzoic acid (PHB) using genetically engineered E. coli. According to the invention, a plasmid is provided which controls the overexpression of chorismate pyruvate lyase, the bacterial enzyme which catalyzes the production of PHB from chorismate. Mutant E. coli selected with a unique two-step screening assay to overproduce chorismate have been transformed with this plasmid, providing a biocatalyst that efficiently converts glucose to PHB.
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申请公布号 |
US6030819(A) |
申请公布日期 |
2000.02.29 |
申请号 |
US19980161364 |
申请日期 |
1998.09.28 |
申请人 |
GENERAL ELECTRIC COMPANY |
发明人 |
AMARATUNGA, MOHAN;LOBOS, JOHN HENRY;JOHNSON, BRUCE FLETCHER;WILLIAMS, ERIC DOUGLAS |
分类号 |
C12N9/88;C12P7/42;(IPC1-7):C12P7/00;C12P7/02;C12P7/22;C12P7/40 |
主分类号 |
C12N9/88 |
代理机构 |
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代理人 |
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地址 |
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