Beta-lactam substrates and uses thereof

摘要

Substrates for beta-lactamase of general formula 1, in which R1 is a moiety, in which a primary amine attached to an aryl carbon of a fluorophore precursor was converted into the nitrogen atom of the beta-lactam ring and the fluorophore precursor is not anthranilic acid when R2 is H; R2 is selected from the group consisting of H and -NR3R4, in which R3 and R4 are selected from the group consisting of H, aliphatic, alkyl, and acyl; and the beta-lactam ring may be cleaved by a beta-lactamase enzyme. Methods of assaying beta-lactamase activity and monitoring expression in systems using beta-lactamase as a reporter gene are also disclosed.