| 摘要 |
PCT No. PCT/GB92/01602 Sec. 371 Date Mar. 1, 1994 Sec. 102(e) Date Mar. 1, 1994 PCT Filed Sep. 2, 1992 PCT Pub. No. WO93/05164 PCT Pub. Date Mar. 18, 1993A marker gene for use in the genetic transformation of plants is driven by a promoter which is capable of acting as a callus-specific promoter, for example in monocotyledonous plants. A suitable promoter naturally drives the expression of a gene encoding a predicted 16.92 kDa protein after wounding and/or callus formation in Asparagus officinalis or an equivalent protein in other members of Liliaceae or Amaryllidaceae. A preferred embodiment is designated the AoPR1 promoter. Under the control of a promoter of the invention, the marker gene (which may for example be an antibiotic- or herbicide-resistance gene) is expressed strongly in wounded tissues and cells and cultured explants, but not constitutively throughout the whole plant. Such promoters may also be used to drive expression of genes in plant tissue culture.
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