differential assay for primary sclerosing cholangistis, type 1 autoimmune hepatitis and ulcerative colitis

摘要

A method of detecting the presence of a perinuclear anti-neutrophil cytoplasmic antibody (p-ANCA) associated with type 1 autoimmune hepatitis in a sample is claimed comprising: a) contacting fixed neutrophils (as defined in the specification) with a sample and a detectable secondary antibody under conditions suitable to form an immune complex of neutrophil, p-ANCA and detachable secondary antibody (as defined in the specification); b) separating unbound secondary antibody from the immune complex and c) detecting the staining pattern of the complex as compared to a control (as defined in the specification).