NUCLEOTIDE SEQUENCES OF LECTINS FRACTIONATED FROM KOREAN MISTLETOE(VISCUM ALBUM COLORATUM

摘要

PURPOSE: Lectins from Korean mistletoe are purified by chromatography and monoclonal antibodies are raised to isolate two different lectins(KML-II U and KML-II L). Gene cloning reveals that two lectins are different from lectins purified from european mistletoe. CONSTITUTION: The invention is disclosed nucleotide sequences of lectins, having molecular weight 68.8 kDa and 56.4 kDa, purified from Korean mistletoe by ammonium sulfate fractionation and Sepharose 4B column chromatography. Two PCR primers( primer 1; 5'-GATIACICATCAIACIGG, and primer 2; 5'-ACIATICGCACIGTIGGTTC) designed from part of amino sequences of purified lectins amplify about 880bp PCR product, cloned into pGEMT vector. Nucleotide sequences of two clones are determined. Nucleotide sequences show that isomers exist like lectins purified from european mistletoe. Purified lectins increase the immune response of immune system and show higher cytotoxic effect than that of lectins from european mistletoe