| 摘要 |
<p>A method and apparatus for determining the fluorescence, luminescence, or absorption of a sample is provided. The sample may either be contained within a cuvette or within one or more sample wells within a multi-assay plate. A combination of a broadband source, a monochromator, and a series of optical filters are used to tune the excitation wavelength to a predetermined value within a relatively wide wavelength band. A similar optical configuration is used to tune the detection wavelength. In one aspect, multiple optical fibers are coupled to the excitation source subassembly, thus allowing the system to be quickly converted from one optical configuration to another. For example, the source can be used to illuminate either the top or the bottom of a sample well within a multi-assay plate or to illuminate a single cuvette cell. Similarly, multiple optical fibers are coupled to the detector subassembly. In another aspect, the excitation light and the detected sample emissions pass to and from an optical head assembly via a pair of optical fibers. The optical head assembly is scanned across one axis of the sample multi-assay plate. The multi-assay plate is mounted to a carriage assembly that scans the plate along a second axis orthogonal to the first axis. In another aspect, an optical scanning head assembly is used that includes mirrored optics for coupling the excitation source to the sample and the emitted light to the detector. An elliptical focussing minor is used to magnify and focus the light projected from an optical fiber coupled to the source subassembly onto the sample. The light from the elliptical mirror passes through an aperture in a second elliptical minor prior to impinging upon the sample. The light emitted by the sample within the sample well is reflected by the second elliptical minor and imaged onto the entrance aperture of an optical fiber coupled to the detector subassembly. The optical axes of both minors are slightly offset from the sample well normal. The minor offset minimizes the amount of light reflected from the meniscus of the sample or the bottom surface of the sample well that enters the detection subassembly. In another aspect, stray light reduction is optimized in order to achieve improved sample emission detection uniformity, particularly emission detection uniformity for an array of fluorescence measurements of a specific sample well. In another aspect, time tags are recorded for samples contained within a multi-assay plate. <IMAGE></p> |
