| 摘要 |
This invention provides methods of amplifying a sequence of interest present within a nucleic acid molecule. In addition, this invention provides a method of determining the nucleotide sequence of a sequence of interest present within a nucleic acid molecule (e.g. GAWTS and RAWTS) which can be used to sequence tissue specific genes (e.g. tsRAWTS) and genes accross species (e.g. zooRAWTS). In addition, this invention provides a method of synthesizing a polypeptide encoded for by a nucleic acid molecule (RAWIT). Further, the subject invention provides a method of determining an internal nucleotide sequence present within a nucleic acid molecule, and a method of determining a terminal nucleotide sequence present within a nucleic acid molecule (e.g. PLATS). Also provided for is a method of determining the nucleotide sequence of sequences present within a nucleic acid molecule which are adjacent to areas of known sequence (e.g ASWATS) and a method of determining the nucleotide sequence of sequences present within a nucleic acid molecule and a method of detecting point mutation or polymorphism (e.g. PASA) which can be used in low cost methods of carrier testing and prenatal diagnosis. Lastly, this invention provides methods for determining the exonic nucleotide sequence of a gene as well as methods of detecting genomic mutations.
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