发明名称 |
Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells |
摘要 |
Disclosed are methods of producing eukaryotic disulfide bond-containing polypeptides in bacterial hosts, and compositions resulting therefrom. Co-expression of a eukaryotic foldase and a disulfide bond-containing polypeptide in a bacterial host cell is demonstrated. In particular embodiments, the methods have been used to produce mammalian pancreatic trypsin inhibitor and tissue plasminogen activator (tPA) in soluble, biologically-active forms, which are isolatable from the bacterial periplasm. Also disclosed are expression systems, recombinant vectors, and transformed host cells.
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申请公布号 |
US6027888(A) |
申请公布日期 |
2000.02.22 |
申请号 |
US19970834516 |
申请日期 |
1997.04.04 |
申请人 |
BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM |
发明人 |
GEORGIOU, GEORGE;OSTERMEIER, MARC |
分类号 |
A61K38/00;C07K14/81;C12N9/72;C12N9/90;C12P21/02;(IPC1-7):C12Q1/68;C12P21/00;C12N1/00;C07H21/04 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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