发明名称 |
A rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction |
摘要 |
A method for determining the existence of duplexes of oligonucleotide complementary molecules is provided whereby a plurality of immobilized oligonucleotide molecules, each of a specific length and each having a specific base sequence, is contacted with complementary, single stranded oligonucleotide molecules to form a duplex so as to facilitate intercalation of a fluorescent dye between the base planes of the duplex. The invention also provides for a method for constructing oligonucleotide matrices comprising confining light sensitive fluid to a surface, exposing said light-sensitive fluid to a light pattern so as to cause the fluid exposed to the light to polymerize into discrete units and adhere to the surface; and contacting each of the units with a set of different oligonucleotide molecules so as to allow the molecules to disperse into the units. |
申请公布号 |
AU5670199(A) |
申请公布日期 |
2000.02.21 |
申请号 |
AU19990056701 |
申请日期 |
1999.08.02 |
申请人 |
THE UNIVERSITY OF CHICAGO |
发明人 |
ANDREI DARIEVICH MIRZABEKOV;GENNADIY MOISEYEVICH YERSHOV;DMITRY YURYEVICH GUSCHIN;MARGARET ANNE GEMMELL;VALENTINE V. SHICK;DMITRI Y. PROUDNIKOV;EDWARD N. TIMOFEEV |
分类号 |
B01J19/00;C12Q1/68;C40B40/06;C40B60/14 |
主分类号 |
B01J19/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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