发明名称 |
REPORTER GENE SYSTEM FOR USE IN CELL-BASED ASSESSMENT OF INHIBITORS OF THE HEPATITIS C VIRUS PROTEASE |
摘要 |
A cell-based assay system in which the detection of the reporter gene activity, or secreted alkaline phosphatase (SEAP), is dependent upon the protease activity of the Hepatitis C virus NS3 gene product. This system can be used to assess the activity of candidate protease inhibitors in a mammalian cell-based assay system. The assay system is simpler than previously described assays due to the use of SEAP which allows the reporter gene activity to be quantified by measuring the amount of secreted gene product in the cell media by monitoring the conversion of luminescent or colorimetric alkaline phosphatase substrate.
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申请公布号 |
CA2328494(A1) |
申请公布日期 |
2000.02.17 |
申请号 |
CA19992328494 |
申请日期 |
1999.08.02 |
申请人 |
AGOURON PHARMACEUTICALS, INC. |
发明人 |
POTTS, KAREN ELIZABETH;JACKSON, ROBERTA LYNN;PATICK, AMY KAREN |
分类号 |
C07K14/18;C07K14/47;C12N5/10;C12N7/00;C12N15/09;C12Q1/02;C12Q1/37;C12Q1/42;C12Q1/70;G01N33/15;G01N33/50;(IPC1-7):C12N15/51;G03C5/00;C07H21/04;G01N33/576;C12N15/66;C12Q1/68;C12N15/86 |
主分类号 |
C07K14/18 |
代理机构 |
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地址 |
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