| 摘要 |
Methods and compositions for assembling active telomerase in vitro and in cells, be they in culture or in vivo, are provided, as are methods and compositions for inhibiting or enhancing telomerase activity through modulation of telomerase assembly. In certain preferred embodiments, methods are provided for the in vitro assembly of a telomerase protein component and a telomerase RNA component, wherein the methods involve the addition of one or more chaperonin molecules, particularly substantially purified or recombinant telomerase chaparonins, which include the proteins hsp40, hsp70, hsp90, p23 and HOP. In such methods, one or more telomerase chaperonins are combined in a reaction mixture that also comprises the catalytic protein and RNA components of telomerase. Screening methods for identifying telomerase assembly and activity inhibitors are also provided, along with methods for stimulating or inhibiting telomerase activity and assembly.
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